Cell Cycle Assay
Labeling cellular DNA can be also be used to analyze the cell cycle phase distribution of cell populations. Labeling may vary as a result of cell-intrinsic factors. Other factors may also affect cell cycle distributions, examples being such things as intake of medical substances and exposure to radiation, or mutation of specific genes, resulting in deviations from the normal cell cycle distribution. Flow cytometry can be used to resolve cells in the different phases of the cell cycle (G0/G1, S-phase adn G2M) with high precision.
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- Fig. 4 shows the histogram of cultured HL-60 cells fixed in ethanol, treated with Rnase, stained with PI, and then analyzed with JSAN.
- Fig. 5 shows cultured Hela cells stained with PI and analyzed with JSAN.
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| Fig. 4: HL-60 Cell Cycle Assay |
Fig. 5(a): Hela Cell Cycle Assay; plot obtained from JSAN software AppSan |
Fig. 5(b): plot obtained from FlowJo |
