About JSAN

Characteristics

Latest Advances in Excitation Optics Design Provide Optimal Excitation Efficiency

• Simple and compact optics offer excellent excitation efficiency.

• A "typical" system could be equipped with very long-lived, very stable 488 nm solid-state and 638 nm semiconductor lasers. Both require only the standard electrical power at your facility and no external cooling.

• The JSAN can be configured with up to four lasers on its standard optical plate, with the use of any three of them simultaneously. (Optional third and fourth lasers are aligned collinearly.)

• Standard JSAN third and fourth laser options include a 375 nm or a 405 nm semiconductor laser.

• The optional 375 nm laser allows analysis and sorting of Hoechst-dyed SP cells, while the 405 nm solid-state laser allow the use of such fluorochromes as Pacific Blue and Pacific Orange.

• Other optional lasers include the latest 534 nm and 561 nm solid-state lasers, with the potential for other lasers, as they are introduced. These laser options enable the optimal use of such recently introduced fluorochromes and fluorescent proteins as DsRed, CFP, and Kaede*.

Compact Laser Optics	488 nm Solid-state Laser (DPSS)	638 nm Semiconductor Laser	375 nm Semiconductor Laser

*Disclaimer: Bay bioscience will communicate with the customer before work commences if there are any concerns about product utility based upon information provided to Bay bioscience. Optional lasers, with the exception of the 375 nm and 405 nm options, lasers are characterized as custom products whose performance has hot yet been characterized.

 

Enhanced Detection Design and User Selectable Filters Expand the Range of Multicolor Analysis

• Optical filsters used in the JSAN are an industry standard size (1-inch or 25 mm round filters).

• Customers may obtain customized filter sets, for their own applications.
  *Bay bioscience cannot guarantee the performance of non-standard optical filters (See TABLE 1 below for the standard JSAN optical filter set).

• The digital signal processor and the digital log conversion circuit convert the detected optical signals into 20-bit signals to be precisely shown on four/six-decade plots.

• The 20-bit digital signal processing has eliminated data dissociation commonly seen in the plot areas close to the axex, ofen the result of fluorescence compensation.

	Detector Layout

	Detector and Intended Dye

 

Improved Optics Expands the Range of Multicolor Analysis and Sorting

• JSAN is controlled from the software Device Parameter control window incorporating a simple tab system. On each tab, users can control the system by entering and specifying optimal parameter type and setting values.

• The Instrument Setting tab accommocates all the controlling parameters for optical detection.

 

8x8 Compensation Matrix for Improved Fluorescence Compensation Calculations and Settings

• Enhanced 8x8 digital matrix compensation circuit enables any type of multicolor analysis that suits your research needs.

• The versatile compensation setting enables compensation of fluorescence excited by different laser beams.

• The parameter names displayed on the matrix allow users to easily recognize fluorochromes or antibodies the compensation is addressing.

	Compensation Matrix
	* The 8x8 matrix describes the situation for the JSAN model with three lasers detecting eight colors offers. A more typical JSAN is likely to be equipped with two lasers, detecting six colors, which would provide 6x6 matrix.